| 1 | Like a Rolling Stone | Bob Dylan | | 4.55 | 6:10 |
| 2 | (I Can’t Get No) Satisfaction | The Rolling Stones | | 4.05 | 3:45 |
| 3 | Imagine | John Lennon | | 4.2 | 3:04 |
| 4 | What’s Going On | Marvin Gaye | | 4.25 | 3:53 |
| 5 | Respect | Aretha Franklin | | 3.9 | 2:26 |
| 6 | Good Vibrations | The Beach Boys | | 4.2 | 3:37 |
| 7 | Johnny B. Goode | Chuck Berry | | 4.2 | 2:40 |
| 8 | Hey Jude | The Beatles | | 4 | 7:08 |
| 9 | Smells Like Teen Spirit | Nirvana | | 4.35 | 5:01 |
| 10 | What’d I Say, Parts 1 & 2 | Ray Charles | | 3.5 | 6:28 |
| 11 | My Generation | The Who | | 4.4 | 3:19 |
| 12 | A Change Is Gonna Come | Sam Cooke | | 3.8 | 3:13 |
| 13 | Yesterday | The Beatles | | 4.75 | 2:07 |
| 14 | Blowin’ in the Wind | Bob Dylan | | 4.55 | 2:48 |
| 15 | London Calling | The Clash | | 4.1 | 3:20 |
| 16 | I Want to Hold Your Hand | The Beatles | | 4.2 | 2:27 |
| 17 | Purple Haze | The Jimi Hendrix Experience | | 4.45 | 2:51 |
| 18 | Maybellene | Chuck Berry | | 3.85 | 2:21 |
| 19 | Hound Dog | Elvis Presley | | | 2:16 |
| 20 | Let It Be | The Beatles | | 4.25 | 4:03 |
| 21 | Born to Run | Bruce Springsteen | | 4.4 | 4:30 |
| 22 | Be My Baby | The Ronettes | | 4.15 | 2:40 |
| 23 | In My Life | The Beatles | | 4.35 | 2:26 |
| 24 | People Get Ready | The Impressions | | 4 | 2:40 |
| 25 | God Only Knows | The Beach Boys | | 4 | 2:51 |
| 26 | (Sittin’ on) The Dock of the Bay | Otis Redding | | 4.45 | 2:42 |
| 27 | Layla | Derek and the Dominos | | 4.45 | 7:07 |
| 28 | A Day in the Life | The Beatles | | 4 | 5:34 |
| 29 | Help! | The Beatles | | 4.2 | 2:21 |
| 30 | I Walk the Line | Johnny Cash and The Tennessee Two | | 4.15 | 2:45 |
| 31 | Stairway to Heaven | Led Zeppelin | | 4.15 | 8:02 |
| 32 | Sympathy for the Devil | The Rolling Stones | | 4.2 | 6:19 |
| 33 | River Deep – Mountain High | Ike & Tina Turner | | 3.5 | 3:39 |
| 34 | You’ve Lost That Lovin’ Feelin’ | The Righteous Brothers | | 3 | 3:44 |
| 35 | Light My Fire | The Doors | | 4.3 | 7:08 |
| 36 | One | U2 | | 4.2 | 4:36 |
| 37 | No Woman, No Cry | Bob Marley & The Wailers | | 3.6 | 3:46 |
| 38 | Gimme Shelter | The Rolling Stones | | 3.95 | 4:33 |
| 39 | That’ll Be the Day | Buddy Holly with The Three Tunes | | | 2:26 |
| 40 | Dancing in the Street | Martha and the Vandellas | | 3.55 | 2:39 |
| 41 | The Weight | The Band | | 4.1 | 4:35 |
| 42 | Waterloo Sunset | The Kinks | | 4.3 | 3:16 |
| 43 | Tutti Frutti | Little Richard | | 4.4 | 2:25 |
| 44 | Georgia on My Mind | Ray Charles | | 4.2 | 3:38 |
| 45 | Heartbreak Hotel | Elvis Presley | | 3.7 | 2:09 |
| 46 | “Heroes” | David Bowie | | 4.6 | 6:10 |
| 47 | Bridge Over Troubled Water | Simon & Garfunkel | | 4.05 | 4:53 |
| 48 | All Along the Watchtower | The Jimi Hendrix Experience | | 4.5 | 4:01 |
| 49 | Hotel California | Eagles | | 4.6 | 6:30 |
| 50 | The Tracks of My Tears | Smokey Robinson and The Miracles | | 4.25 | 2:57 |
| 51 | The Message | Grandmaster Flash & The Furious Five | | 4.4 | 7:11 |
| 52 | When Doves Cry | Prince and The Revolution | | 4.1 | 5:54 |
| 53 | Anarchy in the U.K. | Sex Pistols | | | 4:04 |
| 54 | When a Man Loves a Woman | Percy Sledge | | 4.35 | 2:53 |
| 55 | Louie Louie | The Kingsmen | | 3.8 | 2:45 |
| 56 | Long Tall Sally | Little Richard | | 4 | 2:09 |
| 57 | A Whiter Shade of Pale | Procol Harum | | 4.1 | 4:02 |
| 58 | Billie Jean | Michael Jackson | | 4.1 | 4:54 |
| 59 | The Times They Are A‐Changin’ | Bob Dylan | | 4.1 | 3:14 |
| 60 | Let’s Stay Together | Al Green | | 4.5 | 3:17 |
| 61 | Whole Lotta Shakin’ Goin’ On | Jerry Lee Lewis | | 4.65 | 2:53 |
| 62 | Bo Diddley | Bo Diddley | | 3.25 | 2:45 |
| 63 | For What It’s Worth | Buffalo Springfield | | 4.25 | 2:37 |
| 64 | She Loves You | The Beatles | | 3.7 | 2:21 |
| 65 | Sunshine of Your Love | Cream | | 4.35 | 4:12 |
| 66 | Redemption Song | Bob Marley & The Wailers | | 4 | 3:48 |
| 67 | Jailhouse Rock | Elvis Presley | | 3.55 | 2:28 |
| 68 | Tangled Up in Blue | Bob Dylan | | 3.65 | 5:43 |
| 69 | Crying | Roy Orbison | | 4.65 | 2:47 |
| 70 | Walk On By | Dionne Warwick | | 4.75 | 2:55 |
| 71 | California Girls | The Beach Boys | | 4.5 | 2:45 |
| 72 | Papa’s Got a Brand New Bag | James Brown | | 3.65 | 2:07 |
| 73 | Summertime Blues | Eddie Cochran | | 3.75 | 1:58 |
| 74 | Superstition | Stevie Wonder | | 4.85 | 4:26 |
| 75 | Whole Lotta Love | Led Zeppelin | | 4.45 | 5:34 |
| 76 | Strawberry Fields Forever | The Beatles | | 5 | 4:10 |
| 77 | Mystery Train | Elvis Presley | | 3.4 | 2:26 |
| 78 | I Got You (I Feel Good) | James Brown and the Famous Flames | | 4.7 | 2:47 |
| 79 | Mr. Tambourine Man | The Byrds | | 4.15 | 2:21 |
| 80 | I Heard It Through the Grapevine | Marvin Gaye | | 4.05 | 3:14 |
| 81 | Blueberry Hill | Fats Domino | | 4.65 | 2:22 |
| 82 | You Really Got Me | The Kinks | | 4.25 | 2:14 |
| 83 | Norwegian Wood (This Bird Has Flown) | The Beatles | | 4.55 | 2:05 |
| 84 | Every Breath You Take | The Police | | 4.05 | 4:14 |
| 85 | Crazy | Patsy Cline | | 4.15 | 2:44 |
| 86 | Thunder Road | Bruce Springsteen | | 3.9 | 4:50 |
| 87 | Ring of Fire | Johnny Cash | | 4.25 | 2:37 |
| 88 | My Girl | The Temptations | | 4.5 | 2:45 |
| 89 | California Dreamin’ | The Mamas and the Papas | | 4.45 | 2:40 |
| 90 | In the Still of the Night | The Five Satins | | 4.5 | 3:04 |
| 91 | Suspicious Minds | Elvis Presley | | 4.1 | 4:30 |
| 92 | Blitzkrieg Bop | Ramones | | 4.65 | 2:13 |
| 93 | I Still Haven’t Found What I’m Looking For | U2 | | 4.1 | 4:38 |
| 94 | Good Golly Miss Molly | Little Richard | | 3.5 | 2:09 |
| 95 | Blue Suede Shoes | Carl Perkins | | 4 | 2:16 |
| 96 | Great Balls of Fire | Jerry Lee Lewis | | 4.35 | 1:52 |
| 97 | Roll Over Beethoven | Chuck Berry | | 4.15 | 2:25 |
| 98 | Love and Happiness | Al Green | | 3.7 | 5:04 |
| 99 | Fortunate Son | Creedence Clearwater Revival | | 4.45 | 2:20 |
| 100 | You Can’t Always Get What You Want | The Rolling Stones | | 4 | 7:30 |