| 1 | Down the Road Apiece | Amos Milburn | 2:57 | 1 | Down the Road Apiece | Amos Milburn | 2:57 |
| 2 | Rocket 88 | Jackie Brenston and His Delta Cats | 2:47 | 2 | Rocket 88 | Jackie Brenston and His Delta Cats | 2:47 |
| 3 | Preachin' Blues | Robert Johnson | 2:50 | 3 | Preachin’ Blues | Robert Johnson | 2:50 |
| 4 | Rollin' Stone | Muddy Waters | 3:05 | 4 | Rollin’ Stone | Muddy Waters | 3:05 |
| 5 | Goin' Away Baby | Jimmy Rogers | 2:40 | 5 | Goin’ Away Baby | Jimmy Rogers | 2:40 |
| 6 | The Midnight Special | Lead Belly | 3:02 | 6 | The Midnight Special | Lead Belly | 3:02 |
| 7 | Okie Dokie Stomp | Clarence “Gatemouth” Brown | 4:05 | 7 | Okie Dokie Stomp | Clarence “Gatemouth” Brown | 4:05 |
| 8 | Ay-Te Te Fee | Clifton Chenier | 2:40 | 8 | Ay‐Te Te Fee | Clifton Chenier | 2:40 |
| 9 | Mardi Gras in New Orleans | Professor Longhair & His Shuffling Hungarians | 2:51 | 9 | Mardi Gras in New Orleans | Professor Longhair & His Shuffling Hungarians | 2:51 |
| 10 | Good Golly Miss Molly | Little Richard | 2:08 | 10 | Good Golly Miss Molly | Little Richard | 2:08 |
| 11 | He's Funny That Way | Billie Holiday | 2:38 | 11 | He’s Funny That Way | Billie Holiday | 2:38 |
| 12 | I'm in the Mood | John Lee Hooker | 3:04 | 12 | I’m in the Mood | John Lee Hooker | 3:04 |
| 13 | Jah Is Mighty | Bob Marley & The Wailers | 2:24 | 13 | Jah Is Mighty | Bob Marley & The Wailers | 2:24 |
| 14 | You Win Again | Hank Williams | 2:35 | 14 | You Win Again | Hank Williams | 2:35 |
| 15 | I Can't Believe What You Say | Ike & Tina Turner | 2:00 | 15 | I Can’t Believe What You Say | Ike & Tina Turner | 2:00 |
| 16 | Every Day I Have the Blues | B.B. King | 2:47 | 16 | Every Day I Have the Blues | B.B. King | 2:47 |
| 17 | (They Call It) Stormy Monday | T‐Bone Walker | 3:00 | 17 | (They Call It) Stormy Monday | T‐Bone Walker | 3:00 |
| 18 | Moanin' at Midnight | Howlin’ Wolf | 2:40 | 18 | Moanin’ at Midnight | Howlin’ Wolf | 2:40 |
| 19 | Talkin' to Your Mama | Blind Willie McTell | 3:05 | 19 | Talkin’ to Your Mama | Blind Willie McTell | 3:05 |
| 20 | Bon Ton Roulet | Clarence 'Bon Ton' Garlow | 2:49 | 20 | Bon Ton Roulet | Clarence ‘Bon Ton’ Garlow | 2:49 |
| 21 | Tell It Like It Is | Aaron Neville | 2:39 | 21 | Tell It Like It Is | Aaron Neville | 2:39 |
| 22 | That's What the Blues Is All About | Albert King | 3:50 | 22 | That’s What the Blues Is All About | Albert King | 3:50 |
| 23 | Ruler of My Heart | Irma Thomas | 2:33 | 23 | Ruler of My Heart | Irma Thomas | 2:33 |
| 24 | Pain in My Heart (alternate take) | Otis Redding | 2:20 | 24 | Pain in My Heart (alternate take) | Otis Redding | 2:20 |
| 25 | Baby, Scratch My Back | Booker T. & the MG’s | 3:02 | 25 | Baby, Scratch My Back | Booker T. & the MG’s | 3:02 |
| 26 | Take Me to the River | Al Green | 4:23 | 26 | Take Me to the River | Al Green | 4:23 |
